The release of complete genomic sequences not only offered a huge amount of new data for the molecular biologist, but also impulsed the development of new computational techniques required for the analysis of these data.
Most informatic tools currently available were conceived essentially for the analysis of coding sequences. Non-coding sequences present however a striking interest since they govern the regulation of gene expression.
RSAT offers a series of tools dedicated to the detection of regulatory signals in non-coding sequences. The only input required is a list of genes of interest (e.g. a family of co-regulated genes). From this information, you can retrieve the upstream sequences over a desired distance, discover putative regulatory signals, search the matching positions for these signals in your original dataset or in whole genomes, and display the results graphically in the form of a feature map.
- Analysis of Cis-Regulatory Motifs from High-Throughput Sequence Sets using RSAT (ECCB 2014)
- The RSAT tutorial (online)
- The RSAT command-line tutorial (PDF)
- Build your own virtual RSAT machine.